Abstract: Objective To measurement normal range of the blood routine values and blood biochemical parameters of the GFP Transgenic Nude Mice. To provide a basis for future research. Methods 6~8 weeks male and female 30 mice in each, were used in this experiment. Blood samples were measured using an automatic biochemical analyzer. Compared the differences between GFP Transgenic Nude Mice with BALB/c Nude Mic eand sexes. Results ①The comparison between GFP Transgenic Nude Mice and BALB/c Nude Mice revealed the values of WBC、URE、MCHC、GLU showed very significant difference( P<0.01); HGB、RDW、PLT、UA difference ( P<0.05).②Making a comparison of the male and female of GFP Transgenic Nude Mice, the values of RDW、MCH、MCHC、PLT、G、TC、HDL-TC showed very significant difference( P<0.01); ALB、GLU showed difference ( P<0.05). Conclusion There were some differences to some blood indicators between the male and female GFP Transgenic Nude Mice, the above data could be useful in the biomedical researches.