Objective：to establish human xenograft model on the chick embryo chorioallantoic membrane (CAM) and observe its biological behavior have been reported , however ,there are also some problem on our works, improve some technique to obtain this model which is easy to be duplication. Methods : Human glioma cell line SWO-38 was implanted on the chick embryo chorioallantoic membrane to observe the factors affecting the survival of the transplanted human glioma cells,growth characteristics of the transplanting tumor and the morphological feature . Result : human glioma model was successfully established and it show angiogenesis effects. Conclusions： this model can be easily duplicated and used in biological behavior observation and the research of the drug selection for antineoplastic drugs.