Objective To research the reactivity of the airway of Zmu-1：DHP guinea pig to histamine and the expression of the histamine H1 receptor，and illuminate the relationship between the sensitivity of guinea pig airway and the amount of histamine H1 receptor. Methods Use the method of inhaling histamine and measure resistance of the airway and the dynamics of adaptation of lungs of guinea pig. In vitro，use histamine titration method and measure the contract of airway smooth muscle to the stimulation of histamine. Isotope labeling method is also used to measure the density and equilibrium dissociation constant of histamine H1 receptor. Results The resistance of the airway and the decrease rate of dynamic adaptability of Zmu-1：DHP is sharper than DHP if the concentration is larger than 0.5mg/ml，but the diversity is not significanse（P>0.05）. However，the sensitivity of male increases signaficantly than female in both two strains（P<0.01）. The contract of smooth muscle of Zmu-1：DHP guinea pig is significantly stronger than DHP（P<0.01-0.05）while the concentration of histamine is between 10-5-10-4M. In both two strains，the density and equilibrium dissociation constant of histamine H1 receptor shows almost nothing different（P>0.05）.Conclusion The contract of smooth muscle of Zmu-1：DHP is stronger than DHP to a certain extent of histaming and it is also relative to gender. The sensitivity to histamine in both strains，nevertheless，has no direct relationship to the amount of histamine receptor H1，which demonstrates that airway contraction might be caused by sensitivity of various receptors on the membrane of smooth muscle and the effect of mediums.