【ABSTRACT】 Objective To research the antitumor effection.of MIP-1α and B7-1 gene transfection. on mouse lymphoma. Methods Lentivirus-mediated mouse MIP-1α and B7-1 gene vectors . infected EL-4 cells. The MIP-1α和B7-1 mRNA were identified by RT-PCR and. MIP-1α和B7-1 protein were examined by Western blot. Then the infected EL-4 cells were injected into right armpits of mouse, and observed the development of tumor. The transfected EL-4 cells were infected to tumor-bearing mice, and observe the antitumor effection. Results RT-PCR assay showed that there were MIP-1αand B7-1 mRNA in the EL-4/MIP-1α+B7-1 cells, as well as MIP-1αand B7-1 protein examined by Western blot. MIP-1α and B7-1 group occupied much more time than the control group in the tumor generation, and which mean tumor volume was also smaller than the control group, as well as the rate of tumor formation, however there were no tumor generation in unite group. The average tumor volume，weight and organ involvement rate of MIP-1 α and B7-1 group was significantly smaller than that of the control group ( P < 0.05 ), while the average tumor volume and weight of united group was also significantly smaller than that of MIP-1 α and B7-1 group ( P < 0.05 ). In addition, the mean survival time of unite group was significantly longer than single group, and the control group ( P < 0.05 ). .Conclusion Lentivirus-mediated mouse MIP-1α and B7-1 gene transfection could effectively enhance the anti-lymphoma effection in tumor-bearing mice, and significantly prolonged the average survival time. Which also provided a new idea for the lymphoma gene therapy .