Objective To search for some key genes which account for the different sequelae(chronic woodchuck hepatitis and acute self-limiting woodchuck hepatitis) of woodchuck hepatitis virus infection between neonatal and adult woodchucks. Methods Applying the genome-wide expression microarray, some related genes were selected. With the degenerate primers designed on the iCODEHOP from clustal of some different species, some products were amplified from the woodchuck cDNAs. After the products sequencing done, some accurately designed primers generated from the Primer Premier 5.0, Real time quantitative PCR performed ultimately. Results Compare to the neonatal woodchuck, calcium reabsorption related gene, in the adult woodchuck liver cells DNM1 ( Dynamin 1 ), DNM3 ( Dynamin 3 ) and Prkcc ( Protein kinase C, gamma ) expression rate increased significantly, by 2.65?0.25 times, 1.90 ?.34 times, 2.94 ?.54 times. Conclusion In the endocrine and other factor-regulated calcium reabsorption pathway, these three genes have the most significant changes in mice, as well as in woodchucks at the two time points. Those genes make contribute to the differential calcium concentration, and promote woodchuck hepatitis virus replication indirectly. In a word, these genes could have an effect on the sequelae of woodchuck hepatitis virus infection at different ages.