[Abstract] Objective: To investigate the effects of QDPR on regulating the autophagy of HEK293T cells. Methods：HEK293T cells were transiently transfected with recombinant plasmid DNA rQDPRwt and recombinant plasmid DNA rQDPRmut by calcium phosphate. After 72h, the expression of rat QDPR in 293T cells was detected by Western Blot. Then, the effects of QDPR on autophagy related gene ( including LC3 and Beclin1) were analyzed by RT-PC R, and Western blot was used to monitor the changes in autophagy associated protein level. Results: 1) The recombinant plasmid DNA rQDPRwt and recombinant plasmid DNA rQDPRmut were successfully constructed. 2）The fusion protein can express in HEK293T cell. 3) Compared with control vector group, the mRNA expression of LC3 was significantly up-regulated in rQDPRwt group (p<0.05), and the mRNA expression of Beclin1 showed no significant difference among the 3 groups (p>0.05); 4）The Western blot analysis revealed that LC3-II and Beclin1 increased in rQDPRwt group when compared with control group, and there were no difference of protein levels among the 3 groups. The protein expression of LC3-I，II and Beclin1 showed no significant difference between rQDPRmut group and control group. Conclusion: QDPR may activate the autophagy of HEK293T cells by increasing the expression of autophagy associated genes of HEK293T cells.