Application of Microscopic Examination in Animal Models of intraperitoneal inoculating the Echinococcosis
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The National Basic Research Program of China (973 Program)

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    Abstract:

    Objective This study was designed to establish a simple and rapid methods for observing animal models of echinococcosis qualitatively and quantitatively. Methods Multilocular echinococcus in a dose of 2000 protoscolex / mL were intraperitoneally inoculated in the C. migratorus. Using a simple visual observation and microscopic examination method, the growth of intraperioneal cyst, cyst fluid and protoscoleces was observed in the 10d,15d, 18d, 22d, 39d and 60d after inoculation.. Results Protoscoles were found in the 18d, and cysts became big and many in the 15d,18d and 22d. There was protoscoles blastemal growth in the 39d. Different degree of protoscolex was developed in the 60d. With the extension of the inoculation time, the cyst weight increased. Conclusion The methods for examination of animal models of echinococcosis were established..

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History
  • Received:July 05,2012
  • Revised:August 29,2012
  • Adopted:August 29,2012
  • Online: March 21,2013
  • Published: