Objective We treated simian immunodeficiency virus ( SIV) mac239-infected rhesus macaque monkeys with antiretroviral therapy and examined the characteristics of CD32 expression on resting, activated, and / or memory CD4+ T-cell subsets, to determine their potential as a biomarker of HIV/ SIV reservoirs. Methods Four monkeys chronically infected with SIVmac239 were intramuscularly administrated antiretroviral therapy. Using this animal model, we detected plasma viral loads, CD4⁺T-cell counts, CD4 / CD8 ratios, and the percentage of CD32 expression in different CD4⁺T-cell subsets at different stages. Results Compared with the pre-infection stage, the percentage of CD32 expression in activated naive CD4⁺T cells and HLA-DR⁺ CD4⁺T-cell subsets was significantly increased at the post-infection stage. Moreover, there were no changes in the percentages of CD32 expression in resting CD4⁺T cells, resting naive CD4⁺T cells, resting central memory CD4⁺T cells, resting effector memory CD4⁺T cells, and resting TEMRA CD4⁺T cells. Conclusions This study provides more support for the view that CD32 is not the main biomarker of SIV reservoirs, and provides insight for future AIDS treatment.