Objective In this study, we established a fluorescence quantitative PCR method for rapidly diagnosing a new type of goose-origin astrovirus (GoAstV). Methods After analyzing and comparing the whole-genome sequences of GoAstV downloaded from NCBI, a pair of specific primers and TaqMan probes were designed in its open reading frame-1a conservative region. By optimizing the reaction conditions, a standard curve was established, and the specificity, sensitivity and stability were verified. Results The minimum sample size was 1. 5×10² copies/ μL. The coefficients of variation for the intra-assay and inter-assay detection were < 0. 5% and 4%, respectively, with no specific amplification of other common waterfowl viruses. The clinical application result showed that the detection rate was significantly higher than that of conventional real-time PCR. Conclusions This fluorescence quantitative PCR method exhibited stronger specificity, higher sensitivity and better reproducibility for early detection and quantitative analysis of GoAstV.