Objective To investigate the effects of online teaching in a laboratory animal science course and provide guidance for future teaching activities. Methods A questionnaire survey was conducted among students at a College of Veterinary Medicine in Beijing, China, who had completed an online laboratory animal science course. Results The online laboratory animal science course met the basic teaching Objective established by the College of Veterinary Medicine. However, online teaching alone lacks a practical component; a combined approach using both online and on-site teaching method can provide more robust result. Conclusions These result provide some guidance for the future development of laboratory animal science courses. However, further efforts are needed to more comprehensively establish the teaching model of the course, explore additional teaching method , and improve the quality of instruction.