Establishment of a canine atrial fibrillation model by high-frequency right atrial pacing and evaluation of an implantable electrocardiogram monitor
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(1. The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.2. Kunming Medical University, Kunming 650500.3. Yunnan Fuwai Cardiovascular Disease Hospital, Kunming 650106)

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R-33

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    Abstract:

    Objective In the traditional high-frequency atrial pacing modeling method , we innovatively used fineelectrode wires, advanced catheter control system and implantable electrocardiogram ( ECG) monitor to track atrialfibrillation, to observe the efficiency and safety of the atrial fibrillation model. Methods Twelve adult beagle dogs wererandomly divided into control group (n =6) and atrial fibrillation model group (AF group, n =6). The SelectSecure systemwas used to implant a thin bipolar solid electrode wire 3830, the diameter of which is only 4. 1 Fr, active fixed electrodes inthe AF group, and connect the special atrial fibrillation model pacemaker to establish the atrial high-frequency pacingsystem. After the operation, rapid atrial pacing under the AOO pacing mode was used to establish the atrial fibrillationmodel, and the implantable ECG monitor, Reveal LINQ tracker, was used to track the occurrence of atrial fibrillation inreal time. Color echocardiography was used to examine the echocardiogram of the experimental dogs with a probe emissionfrequency of 2. 5 MHz. The end-systolic atrial area of the left and right atria was measured by echocardiography through thefour apical chambers. The left atrium tissue was observed by light microscopy and electron microscopy. Results Two dogsdied in the experiment and the other 10 dogs completed the experiment. The time it took to establish a successful atrialfibrillation was 10. 63 ± 2. 13 weeks. After establishment of the atrial fibrillation model, the pacemaker atrial stimulationfrequency for the high-frequency atrial fibrillation model was 588. 75 ± 11. 26 beats/ min under the AOO mode. Theimplantable ECG monitor used in this study was accurate and efficient, and can record the AF load dynamically. After theatrial fibrillation model establishment, the left and right atrial areas significantly increased (8. 20 ± 0. 83 cm2 vs. 3. 80 ±0. 08 cm2, P < 0. 05, and 4. 52 ± 0. 44 cm2 vs. 2. 75 ± 0. 96 cm2, P < 0. 001, respectively). Furthermore, the left atrialarea of the AF group was larger than that of the control group (8. 20 ± 0. 83 cm2 vs. 3. 72 ± 0. 15 cm2, P < 0. 05) and theright atrium area was larger than before modeling (4. 52 ± 0. 44 cm2 vs. 2. 78 ± 0. 18 cm2, P < 0. 05). Morphologicalobservation of left atrial tissue sections from the atrial fibrillation model dogs and control dogs indicated that the atrialstructure of the atrial fibrillation experimental dogs had been reconstructed. Conclusions A stable atrial fibrillation modelwas successfully established by high-frequency atrial pacing. The SelectSecure system was used to implant 3830 bipolarsolid atrial pacemaker electrodes. This method accurately implants electrodes and improves the success rate of electrodeimplantation in experimental dogs. Real-time and dynamic monitoring of the atrial fibrillation load with an implantable ECG monitor, Reveal LINQ, improves the efficiency and accuracy of experimental monitoring.

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History
  • Received:September 20,2018
  • Revised:
  • Adopted:
  • Online: October 10,2019
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