Objective To determine the main components of SPF golden hamster milk and compare them withcow milk, human milk, and infant formula milk. Methods Sixty female lactating homsters (1~10 days) were selectedand separated from their offspring for more than 4 h. The milk of the female homsters was collected by an artificial lactationmethod and preserved by freezing. The milk was collected every other day; 2 ml of milk was obtained on average from eachfemale. Testing of the milk composition was performed in accordance with the Chinese National Standards. Results Goldenhamster milk water content was 86. 9 g/100 g, crude protein 6. 95 g/100 g, crude fat 2. 4 g/100 g, lactose 2. 61 g/100 g,and ash 0. 83 g/100 g, with pH 6. 61. Its water content was close to that of cow’s milk and human milk, while its proteincontent was higher. The protein of golden hamster milk is higher than milk?Human milk and infant formula..The fat ofgolden hamster milk is lower than cow’s milk,human milk and infant formula..The lactose of golden hamster milk is lowerthan cow’s milk,human milk and infant formula. Conclusions The main features of golden hamster milk are high protein,low fat, and low sugar, which can provide the basis for the artificial lactation and formulation of sterile golden hamster milk.