Objective To establish an enzyme-linked immunosorbent assay (ELISA) method for detectingantibody to feline panleukopenia virus (FPV) and apply this method for FPV antibody detection in cats. Methods TheFPV antigen was raised in CRFK cells and purified by ultracentrifugation. The purified FPV antigens were coated inmicrotiter plates and goat anti-cat IgG-HRP was used as the detection antibody. After a series of experiments on theoptimization of reaction conditions, the ELISA method was established. Experiments were carried out to evaluate thesensitivity, specificity, repeatability and stability. Results The optimal working densities of the purified FPV antigens andthe enzyme-labeled antibody were 5 μg/ mL and 1∶6000, respectively. The inter-assay and intra-assay average coefficientsof variation were 8. 68% and 7. 14%, respectively. The detection sensitivity was more than 1 ∶5000. There was no crossreactionwith feline herpesvirus type 1 (FHV-1) and feline calicivirus (FCV). Compared with the results of the ELISA kitfrom the European Veterinary Laboratory (EVL), the concordance between the two methods was 90. 7%. Conclusions The established ELISA method exhibites satisfactory duplication, stability, specificity, and sensitivity. This method can be used for the antibody detection of FPV in cats.