Objective To establish a mouse model of pulmonary adenoma, detect its blood biochemistry, and routinely examine its blood, urine, and pathology. Methods PCR confirmed seven B6. 129S4-Krastm^{tm4Tyj/ J-positive} mice. Four of them were used as the experimental group, and three as the control group. Three wild-type mice were used as the control group. Cre adenovirus was applied to the nasal cavity once a week for four times in both the experimental and control groups. After 5 weeks, blood and urine were collected for biochemical examination and routine blood examination. Lung tissue was analyzed for pathology, and quantitative PCR was used to detect Cre expression. Results Four positive mice produced multiple lung adenomas at an average of 4. 25 with a volume of 10. 81 ±6. 38 mm3 . No tumors were found in the control group. Multiple tumors, expansive growth, and clear borders were seen in the lung. The tumor cells were cubic epidermoid and arranged in irregular glandular tubes. No pathological mitosis was seen. Compared with the control group,low-density lipoprotein and average platelet volume were increased significantly. Direct bilirubin in the pulmonary adenoma mice was higher than that in the control group, and high-density lipoprotein was lower than in the control group. These results had high statistical significance. Conclusions A mouse model of pulmonary adenoma is successfully established,and its routine examination of blood and urine,blood biochemistry parameters are determined,and its pathological features are characterized.