Objective To explore the practical value of a modified method for blood collection from the inner canthus of mice using a pipettor. Methods A total of 20 male C56BL/6J mice were divided into two groups, the pipettor group and the capillary group (10 mice in each group). Blood samples were taken 3 times from the inner canthus of the mice with a pipettor or glass capillary, respectively, and the time-consumption and success rate of the two groups were recorded and compared. Results The success rates of both the blood collection methods were 100%. However, in the pipettor group, the blood collection was completed faster than the capillary group with a significant difference (P < 0.05). Conclusions It is a relatively simple and safe method for blood collection from the inner canthus of mice using a pipettor.