Objective To determine the hematological and serum biochemical parameters of 50 healthy African green monkeys, and to analyze the difference of these values between different groups of sex and age (the juvenile group:1-3 years old, the adult group:4-6 years old). Methods Blood samples were obtained from the monkeys in the awake state, and the hematological and serum biochemical parameters of the samples were measured using an automatic blood cell analyzer and blood biochemical analyzer, respectively. Results Among all of the hematologic values, the number of red blood cells (RBC), the amount of hemoglobin (HGB), hematocrit (HCT), and percentages of the neutrophils (NEUT%), lymphocytes (LYMPH%), monocytes (MONO%) and basophils (BASO%) were significantly different between the juvenile group and the adult group (P < 0.05). In the juvenile group, parameters such as the number of the white blood cells (WBC), RBC, HGB, HCT, NEUT%, LYMPH%, MONO% were significantly different between the female and the male monkeys (P < 0.05). As for the adult group, the HCT value was of significant difference, while the other indexes were not significantly different between the female and male monkeys (P < 0.05). Among the serum biochemical values, the quantities of albumin (ALB), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and creatinine (CREA) were significantly different between the juvenile group and adult group (P < 0.05). The amount of CREA of the juvenile females was significantly different from that of the juvenile males (P < 0.05), and the values of total protein (TP) and cholesterol (CHOL) of the adult females were significantly different from those of the adult males (P < 0.05), while the other indexes were not significantly different. Conclusions As a reference, the routine biological data of African green monkeys we have established in this study lay a foundation for the evaluation of their biological properties and further application in related studies.