Objective Laboratory animal practitionres need further management through technical and capacity training. Methods Relying on the Laboratory Animal Center of University and its highly qualified faculty, a high-quality training base for laboratory animal practitioners has been set up. Results Based on the relevant laws and regulations about experimental animals, appropriate training outlines, different training books and courses were prepared and organized for different trainees. Focusing on the detailed and exact experiments, training base has put emphasis on the practical ability and operational skills. Conclusions How to regulate the training for laboratory animal practitioner by means of base training is discussed and tried out in this paper.