Objective To determine the localization of iRhom2 and its mutant proteins of Uncv mice in Vero cells by PKH26 combined with Hoechst 33258 staining. Methods The cell membrane was stained with PKH26, and the nuclei were stained with Hoechst 33258 dye, and observed by laser scanning confocal microscopy. Results It was found that wild iRhom2 was distributed in the cytoplasm, and its iRhom2^mut was present both in cytoplasm and cell nuclei. Conclusions The results of our study suggest that a deletion in N-terminal of iRhom2 affects its subcellular localization.