Objective To investigate the method to prevent the displacement of tissue expander implanted between the panniculus carnosus and deep fasia in rabbits. Method 40 rabbits were divided into two groups randomly:the experimental group and the control group. Through an incision on the back, a pocket was made by dissecting between the rabbit panniculus carnosus and deep fasia. The panniculus carnosus at the pocket boundary were sutured with the deep fascia inside the pocket by interrupted 3-0 sutures. A 50 mL kidney-shaped tissue expander was implanted. The expander was inflated with 10 mL saline after the wound was sutured. 7 days after implantation, black tattoos were made on the skin around the expander to mark the site of expander. Two months after implantation, the expanders in the experimental group were inflated with 10 mL saline weekly to a total of 140 mL. 7 days after implantation the expanders of control group were inflated with 10 mL saline weekly to a total of 140 mL. The incidence of expander displacement, dehiscence of incision, reversal of injection pot and death were recorded.Results There was no expander displacement in the 19 rabbits of experimental group. All the expanders were located within the tattoo area. There was no expander displacement in 11 rabbits of the control group. 4 expanders of the control group moved from the back to belly. The difference between them was statistically significant (P<0.05). The incidence of dehiscence of incision in the experimental group was not significantly lower than that in the control group (P>0.05). Meanwhile there was no significant difference in the incidences of reversal of injection pot, infections and deaths (P>0.05 for all). Conclusions Sutures between the panniculus carnosus and the deep fascia at the pocket boundary asisted by delayed inflating can prevent the displacement of tissue expander implanted between the panniculus carnosus and deep fasia in rabbits, thus to obtain effective expansion.