Objective To investigate the effects of long-term consumption of Fallopia multiflora on mouse hematopoietic system. Methods Forty 10-month old female C57BL/6J mice were equally divided into two groups at random, the control group fed with normal food, and the experimental group, given food with added Fallopia multiflora. After 10 month, the mice were sacrificed, and the peripheral blood, spleen, thymus and bone marrow cells were examined by flow cytometry. Results In the mice fed with Fallopia multiflora, the percentage of B cells in the spleen and CD4⁺ cells in the thymus were increased, and CD8⁺ cells in the thymus and bone marrow hematopoietic stem cells were decreased, among the bone marrow cells, G0 cells were increased, but G1 and G2/S/M cells decreased. Conclusions Long-term proper consumption of Fallopia multiflora can delay the ageing of the hematopoietic system, and sustain its stability.