Objective To study the expression of Growth Hormone (GH) gene in different tissues and different growth stages of Tibet-minipigs. Methods The expression level of GH gene in heart, liver, spleen, lung, kidney, muscle, skin tissue of Tibet mini-pig was analyzed by Real-time PCR in 0 years old (1 days), 0.1 years old (36 days), 0.25 years old (90 days), 0.5 years old (180 days), 1 years old (360 days), 2 years old (720 days), and 3 years old (1080 days). Results The expressions of GH mRNA could be detected in various tissues (heart, liver, spleen, lung, kidney, muscle, skin) in different growth stages (0, 0.1, 0.25, 0.5, 1, 2, 3 years old) in Tibet mini-pigs. The GH gene was most highly expressed in skin in 0, 0.5 years old and most lowly expressed in muscle comparing with other tissues. Moreover, the GH gene was also most highly expressed in lung and liver in 0.25, 1 years old and 0.1, 3 years old, respectively. The expression level of GH gene reached the peak in 0.1 years old among different growth stages. Conclusion The expression of GH gene in Tibet mini-pig showed an obvious temporal and spatial specificity.