Objective To investigate the possibility of utilizing the mixed bacteria liquid and the Escherichia coli (E.coli) liquid to establish the chronic salpingitis model of New Zealand rabbits, respectively. Methods Taken as the study object, the un-pregnant New Zealand rabbits (4~5 years old) were randomly divided into three groups: the normal group, the mixed bacteria experimental group and the E.coli experimental group. The trans-vaginal intrauterine intubation operation was performed for the injection of the mixed bacteria liquid and the E.coli liquid. Visual observation was to evaluate the gross pathological changes of the salpingitis and the pelvic cavity. HE staining and the light microscope were used to observe the micro-pathological changes of salpingitis. Results On the 15^th day after modeling, increased pelvic effusion, dense peritoneal adhesion, interstitial hyperplasia and infiltration of lymphocytes were observed in both experimental groups. Conclusion Through the trans-vaginal intrauterine intubation operation, the chronic salpingitis model of New Zealand rabbits could be successfully established either by using the mixed bacteria liquid or by using the E.coli liquid.