Objective To determine the normal range of hematological and visceral weight parameters of F1 4-week and 8-week old, male and female transgenic CB6F1 mice. The influence of gender and week age on the biochemical parameters was assessed. Methods 4-week and 8-week old CB6F1 mice, half male and half female (n=20 in each group), were weighed alive, dissected to weigh the main viscera, and blood samples were collected to test the physiological and biochemical parameters. Results When 4-week old and 8-week old CB6F1 mice were compared, there were significant differences in 22 parameters (body weight, heart, liver, spleen, left ovary, left testis, right testis, WBC, RBC, HGB, HCT, MCV, MCH, PCT, MPV, PDW, LYM, TP, ALT, ALB, P and TG) (P<0.01 for all), and in 8 parameters (left kidney, right kidney, right adrenal, thymus, left ovary, RDW, MON% and BUN) (P<0.05 for all). When male and female 4-week CB6F1 mice were compared, there were significant differences in 14 parameters (body weight, heart, liver, spleen, lung, left kidney, right kidney, MCHC, LYM, ALT, ALP, GLU, P and CHO) (P<0.01 for all), and in 6 parameters (right adrenal, WBC, PCT, MPV, TP and BUN) (P<0.05 for all). For male and female 8-week old CB6F1 mice, there were significant differences in 15 parameters (body weight, heart, liver, lung, left kidney, right kidney, MCV, PCT, LYM, LYM%, NEUT%, ALT, GLU, P and CHO) (P<0.01 for all), and in 5 parameters (WBC, RBC, MPV, NEUT and TP) (P<0.05 for all). Conclusions The normal range of hematological and visceral weight parameters of 4-week and 8-week old male and female CB6F1 mice are determined. Our study establishes normal detection indexes of CB6F1 mice and provides useful reference for its application.