Objective To obtain the basic growth parameters of a self-established F1 hybrid CB6F1 C57-ras transgenic mouse model, and to provide basic information for commercialization of this mouse model. Methods F1 hybrid mice (CB6F1) were produced by crossing C57-ras heterozygous transgenic (c-Ha-ras^+/-) male mice and wild-type BALB/cJ female mice. The average litter size, weaning rate, sex ratio, growth performance and C57-ras transgenic positive rate were recorded and analyzed. Results The average litter size was eight, weaning rate was 90%, and sex ratio was approximately in accordance with prediction. The average body weight of newborn mice was 1.73±0.05 g. The average body weight of 10-week old c-Ha-ras transgenic female and male mice in CB6F1 background was 24.38±1.74 g and 29.42±1.72g, respectively, which had a significant difference (P<0.01). The c-Ha-ras transgenic positive rate was 46.9%. which was in accordance with genetic rules. Conclusions The F1 hybrid mice (CB6F1) produced by crossing C57-ras heterozygous transgenic (c-Ha-ras +/-) male mice and wild-type BALB/cJ female mice show normal growth performance and development characteristics, and it can be used for large-scale commercial supply.