Objective To investigate the effect of Lycium chinense Mill on hematopoietic reconstruction in mice after radiation injury. Method Female C57BL/6J mice were grouped to 3 groups at random: the control group, with normal food and no treatment, and the other 2 groups, treated with 4.5 Gy irradiation, with normal food or food added with Lycium chinense Mill. After 1 month, the mice were sacrificed and analyzed the peripheral blood, spleen, thymus and bone marrow cells by flow cytometer. Results In the mice fed with Lycium chinense Mill, the percentage of B cells was increased in the peripheral blood, the pre-B and immature B cells were increased, too, and the percentage of hematopoietic stem/progenitor cells was decreased. Conclusion Lycium chinense Mill can promote the mobilization and differentiation of hematopoietic stem/progenitor cells, and accelerate the hematopoietic reconstruction after irradiation injury.