Objective To analyze the survival curves of ASIC3^-/- and TRPV1^-/- mice, and provide a reference for further breeding of these strains of mice. Methods Forty-four ASIC3^-/-mice and sixty-one TRPV1^-/-mice were included in this study. The survival of the mice was observed in normal feeding for 500 days, survival curves were drawn, and their survival was analyzed. Results With the passing of time, the survival probability was decreased in ASIC3^-/-mice and TRPV1^-/- mice. The survival probability of TRPV1^-/-mice was significantly better than that of ASIC3^-/- mice (P =0.004, P<0.01). No significant difference was observed between the males and females of the same strain. Conclusions The survival probability of TRPV1^-/-mice is better than that of ASIC3^-/- mice. There is no significant gender difference in the two strains of mice.