Objective To explore the relationship between mechanical withdrawal threshold (MWTs) and local cutaneous blood perfusion (BP) in rats with acute inflammation induced by carrageenan. Methods Twenty male Sprague-Dawley (SD) rats were randomly divided into control and model groups. The acute inflammatory rat model was established by subcutaneously injecting with carrageenan into the left hindpaw. MWTs were measured by Dynamic Plantar Aesthesiometer 37450 before injection (as base) of carrageenan and at 4 h, 24 h, 48 h, 72 h after carrageenan injection. The local cutaneous BP was detected by Pericam Perfusion Speckle Imager (Pericam PSI) at the time after measuring of MWTs. The above two behaviors were compared and the relationship between them was analyzed. Results MWTs of the model group rats were decreased while BP significantly increased than that in the control group (P<0.01). The MWTs and BP in the model rats showed a negative correlation, especially the correlation index showed significant differences at 4 h and 72 h after carrageenan injection (P<0.01). Conclusions Carrageenan-induced acute inflammation in rats causes significant changes in mechanical pain threshold, which has a negative correlation with local cutaneous blood perfusion.