Objective To evaluate the use safety of a unitary package peracetic acid (PAA) disinfectant through acute and subacute toxicity experiments in rats. Methods This study was conducted according to the Technical Standard For disinfection (Version 2002). In the acute toxicity experiment, 60 healthy Wistar rats were randomly divided into different groups of PAA given by single intragastric gavage. Symptoms of poisoning of the animals were observed to calculate the half lethal dose (LD₅₀).In the subacute toxicity experiment, 40 healthy Wistar rats were randomly divided into three dose groups and negative control group. The rats were given 33-342 mg/kg BW PAA for 28 days. At the end of test, the body weight, the organ-to-body weight ratio and the hematological and biochemical indices were determined, and pathological examination was performed. Results The acute oral LD₅₀ of female rats was 1470 mg/kg BW, and the LD₅₀ of male rats was 1710 mg/kg bw. The results of subacute toxicity test showed that the body weight, the organ-to-body weight ratio and the hematological and biochemical indices had no significant difference between the dose groups and control group. No obvious abnormality was observed in gross anatomy and histopathological examination. Conclusions The results of the acute toxicity test prove that PAA has a low toxicity level, and no significant subacute toxicity was observed at the exposure doses.