Objective To better understand the information of secreted proteins of Tupaia Chinensis.Methods The internet-based software EuSecPred 2.0 and other mapping software were collectively used to predict and analyze the genome-wide secreted proteins. Results There were 279 secreted proteins, accounting for 7.2% of the total proteins encoded by genome sequences. The function and structure of the domain annotation analysis found that they were mainly hydrolases, protein/ion binding proteins and the primary metabolic process-associated proteins. Amino acid composition analysis showed that the signal peptide secreted proteins were mainly composed of hydrophobic amino acids, and there existed some hydrophilic amino acids in the signal peptide cleavage site. Motif analysis revealed no presence of conserved motifs in signal peptide, however, five kinds of motifs existed inside the secreted proteins. Conclusion Genome-wide predictive analysis of secreted proteins of tree shrews is helpful for further study of the regulation of body functions in tree shrews, and for developing mechanism studies and animal models researches.