Objective To investigate the PLCε gene function during growth and development of liver tumor using PLCε knockout mouse models of transplanted liver carcinoma. Methods Fifteen PLCε^+/+ mice were selected randomly as control group,and 15 PLCε^+/+(wild type)and 15 PLC^-/-(knockout genotype)as the model groups I and Ⅱ.H22 liver carcinoma cells were inoculated into the liver parenchyma of mice of the model groups by laparotomy. The mice of control group were injected with saline.Fifteen days after tumor cell inoculation, the rate of tumor formation and tumor volume were observed,and the the tumors were examined by pathology. Results The survival rate of mice in all groups was 100%.In the model group I,the tumor transplantation success rate was 100% and the mean tumor volume was (65.21±5.25)mm³.In the model group Ⅱ,the tumor transplantation success rate was 53.3% and the mean tumor volume was (23.46±3.47)mm³.The average tumor volume of the model group I was significantly greater than that of the model group Ⅱ(P<0.05).Pathological examination confirmed hepatocellular carcinoma in the model groups I and Ⅱ.Conclusion A PLCε gene knockout mouse model of transplanted liver cancer is successfully established, and it demonstrates the property that PLCε gene knockout has an inhibitory effect on liver tumor growth.