Objective To discuss the infective and pathogenic ability of H7N9 avian influenza virus in mammals.Methods The mice model was induced by nose dropping method with influenza virus, by continuous passage in the lung tissue of BALB/c, to get the adaption of seasonal H1N1 in mice and seasonal influenza virus infection model, the lung index and death rate was observed. Results After 9 times continuous passage in the lung tissue of BALB/c, Genome sequencing and alignment indicate that the HA gene, NA gene and PA gene was mutated.Conclusion H7N9 Avian influenza virus A/Anhui/1/2013 gene mutation occurs after continuous passage, but the virμLence and pathogeniceffects of the virus still need further verification.