ObjectiveTo isolated and identify a bacterium from Bama minipigs of diarrhea and the patent medicine were screened by drug susceptibility test.MethodsA bacterium isolated from Bama minipigs was identified by morphological, germiculture, staining, Molecular biology and biochemical characteristics. The resistance test were used to selected the sensitive medicine by 25 drugs. ResultsThe Colibacillus of Bama Minipigs were success isolated and identified as serotype O138. The result of resistance test to 25 drugs showed that the bacterium was sensitive to Penicillin、Streptomycin、Rocephin and Nitrofurantoin, but highly resistant to another drugs that were used.ConclusionThe animal regression test showed that the bacterium have high pathogenicity. The result of this paper would provide the theory basis for prevention and control Colibacillus Infections of related experimental animal.