Objective To compare the serological detection sensitivity of sera against HSV-1 and BV from humans and rhesus monkeys with PR(plaque reduction)and IFA(immunofluorescence assay).Methods The PR assay described in this study was modified from the traditional viral plaque reduction assay by using with 1 mL of DMEM medium containing 1%(weight/volume) of methylcellulose(4000 cP;Sigma,MC),and an improved crystal staining method to achieve better plague formation in HSV-1 infected Vero E6 cells.The neutralizing an...