Objective To establish a technologic platform for generating transgenic mice by lentivirus-mediated delivery of foreign gene(s) into zygotes.Methods\Monomeric red fluorescent protein(mRFP) transgenic mice were generated by subzonal injection of lentivirus harboring mRFP gene into single-cell fertilized eggs of ICR mice to infect fertilized eggs,and subsequently the embryos infected by lentivirus were transplanted into the pseudopregnant mice to attain F0 mice,followed by screening mRFP transgenic mice from ...