Objective To establish PCR testing method that monitor simian foamy virus in laboratory monkeys and related biological products.Methods Designed two series primers of nested PCR according to the region which has high homology among SFV-1,SFV-3,SFV_(CPZ) in pol gene of SFV proviral DNA,then cloned and measured sequence for SFV-1 strain after RT-nestedPCR to determine its accuracy.After tested its specificity and sensitivity,to test peripheral blood lymphocytes of rhesus monkey,passage monkey kidney cells and...