Non-alcoholic fatty liver disease(NAFLD) represents a syndrome of liver disease encompassing steatosis(fatty change),non-alcoholic steatohepatitis(NASH) and cirrhosis in the absence of alcohol abuse.NAFLD is now recognized as the most common type of liver disease and is emerging as a common medical problem.Along with the research and production of anti-fatty liver effects drugs,the animal models are also well developed.In recent years,NAFLD animal models have been successfully established in many species such as rat,sand rat,mice,rabbit,and piglets induced by diet,total parenteral nutrition,and methionine choline-deficient.These models provide new opportunities to study the pathogenesis and treatment of metabolic syndrome associated with NAFLD/NASH.Each animal model has its advantages and disadvantages.Using reasonable animal model may make it better to carry on the laboratory and clinical study of NAFLD/NASH.In this paper,we reviewed the progress in studies of preparation in animal models of NAFLD/NASH.