Non-human primates, which are extensively used in biomedical researches, play an important role in the study of physiology, pathology, virology, pharmacokinetic, toxicology, reproduction physiology, immunology, genetics, gene engineering drug and biological medicine and so on. Therefore, the animal welfares should be emphasized in research works. According to our practices and demand of animal welfares, this paper summarized that the veterinarian and animal nursing act in the welfares of non-human primates, which includes disease monitor, diagnosis, therapy, prevention, experimental operation, catching, securing, composing, anaesthesia, odynolysis and euthanasia etc.