Objective The effect factors on blood glucose of KK/Upj-A^y/J mice with nutrition, age and sex. Methods Tail blood with experimental group mice and comparative group mice were measured by blood glucose detector. Results Either blood glucose level or ratio to all mice, experimental group were significantly highter than that of comparative group. On comparative group, blood glucose level is low on 8 months old. On the other hand, experimental group mice in both sex, age have a few bearing on blood glucose level. The sex has almost not an influence on all animal experiment. Conclusion I .nutrition components is main factors which affect blood glucose at same genetic background with A^ygene; 2.Another effect factor is the age of the mice; 3.The sex is not an important factor to blood glucose in the KK/Upj-A^y/J mice.