Objective To establish a hyperlipidemia animal model induced by high-fat diet,and observe the dynamic variation of the serum lipid levels at different time.Methods The C57BL/6J mice were randomly divided into 2 groups.The control group was fed with standard diet,the model group fed with high-fat diet.The serum TG,TC,HDL,LDL,ALT,albumin,and BUN were assayed at 0,1,2,3,4,5,7,9,1 1,17 weeks.Some mice were killed and the heart,liver and kidney were taken and HE-stained sections were prepared for histopathological examination.Then high-fat diet of the model group was changed to standard diet.After 4 weeks all mice were killed and the serum contents of each substance were detected.Results The levels of lipid and ALT in serum rose rapidly after 1 week of high-fat diet,and stay at a high level throughout the duration of 3-11weeks,then decreased a little.Pathological changes were observed in the heart and liver.The serum lipid and ALT levels decreased rapidly to normal level after stopping high-fat diet.Conclusion Feeding with high-fat diet can induce hyperlipidemia in mice rapidly and reliably.