Objective To establish an optimal model of medulla-suppressed anemia mice for curative observation. Methods 80 normal BALB/c mice were distributed randomly into 10 groups. The medulla haematogenous effect was compared among the normal group, the irradiation group, the injection cyclophosphamide group, the irradiation and injection cyclophosphamide combation group and the irradiation, cyclophosphamide and chloramphenicol combation group. Results Compared with normal group, the hemogram and bone marrow cell in other groups all decreased singificantly. Through analyzing death rate and amount of whole blood cell, effect of the irradiation and cyclophosphamide combation group was the most remarkable. Conclusion The irradiation and cyclophosphamide combation group is the best model for suppressing hematopoiesis of bone marrow in anemia mice.