Objective To prepare BaMa minipig's acellular dermal substitute in order to ultilize it into xeno-transplant in clinical use.Methods Epidermis of BaMa minipig's skin was removed by 0.1% dispaseII or 0.25% typsin for one night,then dermis was treated with 0.5% triton X-100 for 24h,succussed or not succussed.The prepared acellular dermal matrix was examined macroscopically with gross observation,light microscopy,bacteris culture test and cytotoxicity test.Results Epideris could be removed thoroughly either in 0.1% dispaseII or 0.25% typsin for one night,but the basal memberane was integrity in 0.25% typsin and in 0.1% dispaseII the basal memberane was not integrity.Cellular components were elimated thoroughly treated by succuss in 0.5% triton X-100 for 24h.There were not any bacteria and ctyotoxity.Conclusion Prepared BaMa minipig's acellular dermal substitute had no cellular components,bacteria and cytotoxity,which would be an ideal dermal substitute.