Objective To establish a rabbit model of the cardiopulmonary bypass(CPB) flushed without blood.Method Thirty New Zealand rabbits were included in this experiment.The CPB procedure was established via right subclavian artery and right atrium cannulation.The blood-gas parameters were examined before and after CPB.Results The rabbit cardiopulmonary bypass model was established successfully.The results of arterial blood gas analysis were satisfactory.No hemorrhage and operation death occurred in this experiment.Conclusion A rabbit model of cardiopulmonary bypass flushed without blood has been established and showed to be feasible for related pathophysiological research work.