Objective To develop a method with high sensitivity,specificity and rapidity to detect pathogenic dermatophytes in laboratory animals.Methods One fragment of CHS1 gene was selected as universal primer of dermatophytes in this PCR amplification.Otherwise,a random primer FM1 was also used to amplify three dermatophytes species standard strain: Trichophyton mentagrophyton,Microsporum gypseum,Microsporum canis.Results There were distinguishable fingerprinting patterns among three dermatophytes species.Conclusion The test could detect some common dermatophytes species of laboratory animal rapidly and specifically.