Objective To establish an model of experimental acute pancreatitis(AP) in rats by operational method.Methods Wistar rats were divided randomly into three groups.The end of bile duct was ligated in the test group;in the control group placket of duodenum was ligated one centimeter far and near to the bile duct;the normal group as a control:the normal Wistar rats.Test and control groups rats were phlebotomized at 10,24,48h respectively,for detection of the amylase concentration,calcium and blood sugar.At the same time,the pathological change and the histological change were examined.Results The level of amylase concentration of test and control groups increased significantly,and higher than the normal group as a control;the level of calcium reduced significantly,but blood sugar had no remarkable change.Conclusion Biochemical analysis and histological examination demonstrated that the AP model was successfully constructed.;