Objective To observe the changes of aging data of Wistar rats in the subacute aging model induced by the subcutaneous injection with D-galactose.Methods 20 male Wistar rats were divided randomly into two groups.Each group contained 10 rats for the control or aging model.The control rats were injected subcutaneously with 10 ml PBS/kg each day,and the rats of the aging model with 48 mg D-galactose/kg each day.After 42 days with continuous injection,the blood specimens were collected for the detection of the data relative to the aging. Results Compared with the control , the weight of the aging model rats increased slowly. The thymus of these rats shrivelled clearly,and their weight decreased as well (P<0.01).In addition,the superoxide dismutase(SOD) activity in the serum of these aging rats declined obviously(P<0.01). Conclusion The aging model could be established by the mimic aging reaction induced with D-galactose.