Objective A laboratory method of aerosol generation is studied for animal inhalation toxicity experiment. Method\ Through the atomization of solution and suspensions with compressed air used to generate aerosol, air pressure, flow rate and aerosol concentration are measured. Results\ When air pressure is 2-3 atm, flow rate of three\|hole\|nebulizer is 13 67-18 67 L/min, aerosol concentration is about 10000 mg/m 3, and flow rate of one\|hole\|nebulizer is 10 67-15 50 L/min, aerosol concentration is about 5000 mg/m 3. Conclusion\ According to the given condition, one\|hole\|nebulizer and two diluters are chosen for the purpose.\;