Objective To investigate the curative effect of ethanol extract from propolis (EEP) in phthiriasis of animal.Methods Pharmacodynamic screening test and curative effect test of EEP in sucking lice of rats were done at four different concentration (5g/L, 7 5g/L, 10g/L and 15g/L).Results 10g/L EEP is best ( P <0 01) to kill sucking lice of rat,and to treat phthiriasis of rat.Conclusion Propolis is a natural,cheap,nonpoisonous and easy to gain agent for prevention and treatment of animal phthiriasis.