Ovarian tissue cryopreservation is being developed as a method for preserving the ovarian function of young women at risk of early ovarian failure and young cancer patients. Now this research is still at animal experimental stage. Based on a lot of animal experiments, scientists have developed two kinds of cryopreservation programme:slow cooling-fast thawing method(two step method),fast cooling-fast thawing method.The cryoprotectants have also been investigated and lots of methods to evaluate the viability of frozen-thawed ovarian tissue produced. Recently,a lamb was born after orthotopic transplantation of frozen ovine ovarian tissue. We believe that human being will be benefited from this work.