Abstract: Objective　To determine the blood physiological and biochemical indexes in the inbred SHANXI MU strain of spontaneous type 2 diabetes (T2DM) Chinese hamsters. Methods　Chinese hamsters with spontaneously developed T2DM and normal hamsters (n=10 hamsters per group), aged 12 months, were selected for the study. Fasting blood samples were collected and 15 physiological parameters and 16 biochemical indicators were analyzed using a Sysmex XT automated hematology analyzer and Hitachi fully automatic biochemical analyzer. Results　The white cell count, red cell count, platelet count, hemoglobin level, alanine transaminase, aspartate transaminase, glutamate, total cholesterol, triglycerides, and uric acid all differed significantly between the diabetic and control groups (P<0.05). Conclusions The change of blood physiological and biochemical indexes in the Chinese hamster spontaneous T2DM model were in line with the trend in human T2DM incidence, thus providing basic data for the application of this model.

Abstract:Objective To study the effect of age and sex on physiological and biochemical indexes of SJ5?SPF chickens at the age of different weeks. Methods Blood biochemical indexes of SJ5?SPF chickens at the age of different weeks were measured using an automatic blood analyzer, including 19 items: ALT, ALP, GGT, AST, LDH, TP, GLB, ALB, TB, DB, IBIL, GLU, TG, TCH, BUN, CRE, K+ , Na+ , and Ca2 + . At the same time, the body temperature (T), respiratory frequency (R), heart rate (HR), diastolic pressure (DBP) and systolic pressure (SBP) of the SJ5?SPF chickens were measured using a RM6240C multi?channel physiological signal acquisition and processing system. Results (1) Among the physiological parameters, the body temperature, respiratory rate, heart rate, diastolic blood pressure and systolic blood pressure showed significant differences between the 4?week old chickens and the chickens at ages of 20, 25, and 25 weeks ( P < 0.05), but there were significant differences between the males and females at the same weeks of age except body temperature. The diastolic blood pressure was only significantly different in the 40?week old male and female chickens ( P < 0.01). The heart rates showed significant differences between the male and female chickens at 4, 20 and 25 weeks of age ( P < 0.01). The respiratory rate and systolic blood pressure in the 4?week old male and female chickens were significantly different ( P < 0.05). (2) The 19 blood biochemical indexes showed significant differences between the male and female chickens: the GGT, ALT, AST, TB, DB, IBIL, GLU, BUN and Na+ at an week?age ( P < 0.05), the LDH, TP, GLB, ALB and CRE at two different week?ages ( P < 0.05), the ALP, TG and Ca2 + at three different week?ages ( P < 0.05), and the TCH and K+ at four different week?ages ( P < 0.05). Conclusions The above results can provide a scientific basis for diagnosis of SJ5?SPF chicken disease, animal quarantine and other related research.

Abstract:Objective To compare the difference in physiological and biochemical parameters between Primary and the First Generation in Ningxiang pig. Methods The conventional method for the determination of 18 physiological indexes and 13 blood biochemical indexes of Ningxiang pig F0 generation and F1 generation. Results In F0 and F1 generation, 13 blood physiological indexes such as WBC, RBC, HGB, HCT has significant difference（P<0.05）; Some hematological parameters such as TBIL, BUN, TP, ALB / GLB, GLU has significant difference ( P< 0.05 ) ; Other indicators were not statistically different. Conclusions The results showed that the difference of blood physiological and biochemical parameters between two Generations in Ningxiang pig is significant. Ningxiang pig can be raised to be laboratory animal.