Objective To investigate the effect of Dendrobium nobile Lindl (DNL) on Caenorhabditis elegans (C. elegans) model of Parkinson"s disease, 6-OHDA was used to induce C. elegans model of Parkinson"s disease(PD), and Dendrobium nobile was used to interfere with C. elegans. Methods C. elegans NL5901 and 6-OHDA-induced N2, BZ555, PD4521 and BC7272 were treated with DNL of 7.5, 15 and 30mg/L. The survival rate, basal slowing response rate, α-synuclein aggregation(α-syn), dopaminergic neurons(DNs), mitochondrial distribution density of body wall muscle cells and protein levels in the membrane were observed. In addition, the activity of SOD and GSH in 6-OHDA-induced N2 was detected to explore the effect of DNL on the anti-oxidative stress ability of Parkinson disease C. elegans models. Results Compared with the PD model group, the DNs fluorescence intensity of the nematodes treated with DNL and L-DOPA was significantly increased (P<0.001), α-syn aggregation was significantly decreased (P<0.05 or P<0.001), the basal slowing rate was increased (P<0.05 or P<0.001), mitochondrial density was increased (P<0.001), mitochondrial intima protein content was increased (P<0.05 or P<0.001), SOD(P<0.05) and GSH content are also increased. The lifespans of N2 wild type C. elegans and PD C. elegans models were prolonged after DNL treatment (P<0.05 or P<0.001). Conclusions A certain concentration of DNL can effectively improve the paralysis of C. elegans model of Parkinson"s disease, improve the degeneration of dopaminergic neurons, reduce the accumulation of α-syn and suppressed the loss of DNs. Overall, DNL treatment increase the ability of anti-oxidative stress, and slow down the aging process of C. elegans. These results provide reference for the screening of effective drugs for Parkinson"s disease.